Scientific Equipment Source
1501 Harwood Ave N
Ajax, ON, L1T 4T9
Canada
Tel: 905-231-0930
Fax: 905-231-0293

Let us match any price. Please let us know if you got better deal somewhere else we might be able to offer you something better.
Leading supplier of refurbished and used laboartory equipment. Systems include Agilent, Hewlett Packard HP 1050, HP 1090, HP 1100, HP 5890, (series II and plus), HP 6890, ChemStation with computer, Waters HPLC with millennium software. Systems are completed and setup to your specific application and include guarantee period.

We also have wide range of ( used and refurbished ) spectrophotometers on stock. Currently we can offer Hewlett Packard HP 8452A Diode Array UV Visible Spectrophotometer, VG Plasma Quad, Mass Spectrophotometer, Atomic Absorption and wide range of other spectrophotometers.

HPLC is a popular method of analysis because it is easy to learn and use and is not limited by the volatility or stability of the sample compound. Modern HPLC has many applications including separation, identification, purification, and quantification of various compounds. Prior to the 1970's, few reliable chromatographic methods were commercially available to the laboratory scientist. During 1970's, most chemical separations were carried out using a variety of techniques including open-column chromatography, paper chromatography, and thin-layer chromatography. However, these chromatographic techniques were inadequate for quantification of compounds and resolution between similar compounds. During this time, pressure liquid chromatography began to be used to decrease flowthrough time, thus reducing purification times of compounds being isolated by column chromatogaphy. However, flow rates were inconsistant, and the question of whether it was better to have constant flow rate or constant pressure was debated. (Analytical Chem. vol 62, no. 19, oct 1 1990).

High pressure liquid chromatography was developed in the mid-1970's and quickly improved with the development of column packing materials and the additional convenience of on-line detectors. In the late 1970's, new methods including reverse phase liquid chromatography allowed for improved separation between very similar compounds.

By the 1980's HPLC was commonly used for the separation of chemical compounds. New techniques improved separation, identification, purification and quantification far above the previous techniques. Computers and automation added to the convenience of HPLC. Improvements in type of columns and thus reproducibility were made as such terms as micro-column, affinity columns, and Fast HPLC began to immerge.

The past decade has seen a vast undertaking in the development of the micro-columns, and other specialized columns. The dimensions of the typical HPLC column are: XXX mm in length with an internal diameter between 3-5 mm. The usual diameter of micro-columns, or capillary columns, ranges from 3 µm to 200 µm. Fast HPLC utilizes a column that is shorter than the typical column, with a length of about 3 mm long, and they are packed with smaller particles.

Currently, one has the option of considering over x# types of columns for the separation of compounds, as well as a variety of detectors to interface with the HPLC in order to get optimal analysis of the compound. We hope this review will provide a reference which all levels of HPLC users will be able to find quick answers to their HPLC problems.

Although HPLC is widely considered to be a technique mainly for biotechnological, biomedical, and biochemical research as well as for the pharmaceutical industry, these fields corrently comprise only about 50% of HPLC users.(Analytical Chem. vol 62, no. 19, oct 1 1990). Currently HPLC is used by a variety of fields including cosmetics, energy, food, and environmental industries.

Our site is divided into several sections;


Scientific Equipment Source is a leader in Sales and Services of quality reconditioned and new laboratory and scientific equipment. We can also offer wide range of instruments, which has not been refurbished, and it can be sold as is (working), please ask for availability.
We carry a wide range of new laboratory equipment and scientific apparatus that has been rebuilt and reconditioned using trained technicians to save you money and give you confidence in our product.
We ship worldwide, standard skid one cubic meter about 200 Kg would cost between 400 – 700 USD depending on destination. This price includes only the delivery to a port by ocean does not include any local taxes and fees.
For USA and Canada about 200 - 300 USD.
At Scientific Equipment Source our products come refurbished and with a warranty. The warranty period depends on the type of equipment being purchased. All warranties can be extended from the standard purchase warranty to a longer period of time if desired and also preventative maintenance packages can be added as well.